Asian Journal of Research in Chemistry

B Sandeep Kumar Naik ¹, N Duganath ¹, S Rubesh Kumar ¹, N Devanna ¹

Affiliations
1 Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapur -515001, Andhra Pradesh, IN

Abstract

Hesperidine, a bioflavonoid, is an abundant and inexpensive by-product of Citrus cultivation. A deficiency of this substance in the diet has been linked with abnormal capillary leakiness as well as pain in the extremities causing aches, weakness and night leg cramps. No signs of toxicity have been observed with the normal intake of hesperidine or related compounds. Hesperidine was extracted from the dried peel of Citrus limetta which belongs to the family Rutaceae. An amount of 1kg air-dried and powdered peel was extracted to get 10gms of hesperidine which was a white brownish solid. Analysis of isolated hesperidine along with standard Hesperidine and marketed ayurvedic polyherbal formulation were identified by using IR spectroscopy, UV Spectroscopy, Thin Layer Chromatography (TLC) and UFLC. IR characterization showed that the standard, isolated and ayurvedic formulation were same. In UV spectroscopy all the components showed specific absorbance at 283.5nm. The Rf values for isolated, standard and marketed ayurvedic formulation were found to be 0.710, 0.708 and 0.702 respectively. A reversed phase UFLC method was also developed for determination and quantification of hesperidine. The method involved the use of a Phenomenex Luna 5u C18 column, a mixture of water-Acetonitrile-methanol (50:25:25 v/v/v) as a mobile phase in an isocratic mode at a flow rate of 1 ml/min and UV detection at 283.5 nm.

Keywords

Extraction, Characterization

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S Rubesh Kumar ¹, G Usha Sree ¹, K Jayanthi ¹, B Naga Malleswara Babu ¹, N Duganath ¹, N Devanna ¹

Affiliations
1 Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapur -515001, Andhra Pradesh

Abstract

A simple, sensitive, and precise High performance liquid chromatographic method for the analysis of Naproxen and Pantoprazole has been developed and validated for the determination of compounds in commercial pharmaceutical products. The compounds were well separated on a BDS Hypersil C-18 reversed-phase column by use of a mobile phase consisting of Acetonitrile and Mixed Phosphate buffer (pH 6.92) in 45:55(V/V%) ratio, at a flow rate of 1.0 mL/min with detection wavelength at 290 nm. The linearity ranges were 20-120μg. The correlation coefficient was found to be 0.997 and 0.995 for Naproxen and Pantoprazole respectively. The recovery amount was in the range of 99.67-101.39%. The high recovery and low relative standard deviation confirms the suitability of the method for determination of Naproxen and Pantoprazole in capsule dosage forms.

Keywords

Naproxen, Pantoprazole

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V Hima ¹, S Rubesh Kumar ¹, N Duganath ¹, N Devanna ¹

Affiliations
1 Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapur, Andhra Pradesh, IN

Abstract

An Ultra Fast liquid chromatographic method has been developed and validated for simultaneous estimation of ascorbic and gallic acid in both API and Ayurvedic formulation. Chromatographic separation of compounds was carried out with C8 column by using a mobile phase of methanol: phosphate buffer, PH 3.0 (1: 5) at a flow rate of 1.0 ml/ min. UV detection was performed using PDA detectors at 264 nm. The method was validated for accuracy, precision, linearity, LOD, LOQ and robustness in accordance with ICH guidelines. Amounts of ascorbic and gallic acid detected in capsule were 99.20% and 99.45%. Total run time was below 3 min, ascorbic and gallic acid was eluted with retention times of 1.541 and 2.591 min respectively. Validation revealed that the method is specific, accurate, precise, reliable and reproducible. Calibration plots were linear over the concentration ranges 1-9 μg/ ml for ascorbic acid and for gallic acid, respectively. Limits of detection were 0.0382 and 0.14811 μg/ ml and limits of quantification were 0.1159 and 0.4488 μg/ml for ascorbic and gallic acid, respectively. Recovery was 99.60 - 100.28% and 100.26 -101.35% for ascorbic and gallic acid, respectively. The stress degradation studies were performed for both API and Ayurvedic as per ICH guidelines, the degradation was observed in oxidative, photolytic, hydrolytic degradation under acidic, alkaline conditions and dry heat induced studies. The proposed method is rapid, simple and also it can be applied for the routine analysis of herbal formulations.

Keywords

Ascorbic Acid, Gallic Acid, UFLC, Stress Degradation Studies

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